Symbiotic photobioreactor using immobilized microalgae-yeast consortium for saccharomyces cerevisiae and chlorella vulgaris biomass production
Corressponding author's email:
linhvtk@hcmute.edu.vnKeywords:
Saccharomyces cerevisiae, Chlorella vulgaris, biomass production, symbiotic culture, photobioreactor, photosynthetic aerationAbstract
A photobioreactor for symbiotic growths of Saccharomyces cerevisiae and Chlorella vulgaris was developed. In this photobioreactor, yeast cells were freely suspended in medium while microalgal cells were entrapped in alginate beads in the same medium for separate biomass production. The symbiotic relationship was demonstrated as reflected by the photoautotrophic growth of C. vulgaris using CO2 provided by S. cerevisiae and the biodegradation of 2000 mg/L glucose by S. cerevisiae utilizing photosynthetic O2 produced by C. vulgaris. Yeast biomass productivities were 67.75 mg/L-h, 74.70 mg/L-h and 68.53 mg/L-h in three successive 8-hour photo-aeration cycles, significantly higher than that obtained in non-aerated yeast culture. Microalgal cell concentration also increased 84% after 24 hours of cultivation in the symbiotic photobioreactor, significantly higher than those grown in the medium without external carbon source. Enhanced yeast biomass productivity was obtained in symbiotic medium supplemented with 4000 mg/L glucose. The highest yeast biomass productivity of 99.82 mg/L-h was obtained at an initial microalgal concentration of 110.52 mg/L.
Downloads: 0
References
Gómez-Pastor, R., Pérez-Torrado, R., Garre, E., & Matallana, E. (2011). Recent advances in yeast biomass production. In Biomass-Detection, production and usage. InTech.
Vieira, É. D., Andrietta, M. D. G. S., & Andrietta, S. R. (2013). Yeast biomass production: a new approach in glucose-limited feeding strategy. Brazilian Journal of Microbiology, 44(2), 551-558.
Di Serio, M., De Alteriis, E., Parascandola, P., & Santacesaria, E. (2001). A general kinetic and mass transfer model to simulate the baker’s yeast growth in bioreactors. Catalysis today, 66(2-4), 437-445.
Pérez-Torrado, R., Gamero, E., Gómez-Pastor, R., Garre, E., Aranda, A., & Matallana, E. (2015). Yeast biomass, an optimised product with myriad applications in the food industry. Trends in Food Science & Technology, 46(2), 167-175.
Najafpour, G. D. (2007). Single-Cell Protein. Biochemical Engineering and Biotech.
Humbird, D., et al. (2017). Aeration costs in stirred-tank and bubble column bioreactors. Biochemical Engineering Journal, 127: 161-166.
Stanbury, P. F., et al. (1995). CHAPTER 12 - Fermentation Economics. Principles of Fermentation Technology (Second Edition). P. F. Stanbury, A. Whitaker and S. J. Hall. Amsterdam, Pergamon: 331-349.
Chen, S. L. and F. Gutmanis (1976). Carbon Dioxide Inhibition of Yeast Growth in Biomass Production. Biotechnology and Bioengineering, 18(10): 1455-1462.
Chen, C. Y., Yeh, K. L., Aisyah, R., Lee, D. J., & Chang, J. S. (2011). Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresource technology, 102(1), 71-81.
Pandey, A., Lee, D. J., Chisti, Y., & Soccol, C. R. (Eds.). (2013). Biofuels from algae. Newnes.
Lehr, F. and C. Posten (2009). Closed-photobioreactors as tools for biofuel production. Current Opinion in Biotechnology, 20(3): 280-285.
Spolaore, P., et al. (2006). Commercial applications of microalgae. Journal of Bioscience and Bioengineering, 101(2): 87-96.
Kück, U., Frankenberg‐Dinkel, N. (2017). Biotechnology. De Gruyter
Meyer, H. P., & Schmidhalter, D. (2014). Industrial scale suspension culture of living cells. John Wiley & Sons.
Ugwu, C. U., Aoyagi, H., & Uchiyama, H. (2008). Photobioreactors for mass cultivation of algae. Bioresource technology, 99(10), 4021-4028.
Brennan, L., & Owende, P. (2010). Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products. Renewable and sustainable energy reviews, 14(2), 557-577.
Chisti, Y. (2007). Biodiesel from microalgae. Biotechnology Advances, 25(3): 294-306.
Peng, L., et al. (2013). Evolution, detrimental effects, and removal of oxygen in microalga cultures: A review. Environmental Progress & Sustainable Energy, 32(4): 982-988.
Acién, F. G., et al. (2012). Production cost of a real microalgae production plant and strategies to reduce it. Biotechnology Advances, 30(6): 1344-1353.
Gomez, J. A., et al. (2016). From sugars to biodiesel using microalgae and yeast. Green Chemistry, 18(2): 461-475.
Santos, C. A. and A. Reis (2014). Microalgal symbiosis in biotechnology. Applied Microbiology and Biotechnology, 98(13): 5839-5846.
Cheirsilp, B., Suwannarat, W., & Niyomdecha, R. (2011). Mixed culture of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for lipid production from industrial wastes and its use as biodiesel feedstock. New biotechnology, 28(4), 362-368.
Xue, F., et al. (2010). A new strategy for lipid production by mix cultivation of Spirulina platensis and Rhodotorula glutinis. Appl Biochem Biotechnol, 160(2): 498-503.
Zhang, Z., et al. (2014). Synergistic effects of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for enhancement of biomass and lipid yields. Bioresource Technology, 164: 93-99.
Shu, C. H., Tsai, C. C., Chen, K. Y., Liao, W. H., & Huang, H. C. (2013). Enhancing high quality oil accumulation and carbon dioxide fixation by a mixed culture of Chlorella sp. and Saccharomyces cerevisiae. Journal of the Taiwan Institute of Chemical Engineers, 44(6), 936-942.
Elnashar, M. M., Yassin, M. A., Abdel Moneim, A. E. F., & Abdel Bary, E. M. (2010). Surprising performance of alginate beads for the release of low‐molecular‐weight drugs. Journal of applied polymer science, 116(5), 3021-3026.
Moreno-Garrido, I. (2008). Microalgae immobilization: current techniques and uses. Bioresource technology, 99(10), 3949-3964.
Smidsrød, O., & Skja, G. (1990). Alginate as immobilization matrix for cells. Trends in biotechnology, 8, 71-78.
Praveen, P., & Loh, K. C. (2015). Photosynthetic aeration in biological wastewater treatment using immobilized microalgae-bacteria symbiosis. Applied microbiology and biotechnology, 99(23), 10345-10354.
Cheng, N. G., Hasan, M., Kumoro, A. C., Ling, C. F., & Tham, M. (2009). Production of ethanol by fed-batch fermentation. Pertanika J Sci Technol, 17(2), 399-408.
Andersen, R. A. (Ed.). (2005). Algal culturing techniques. Elsevier.
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009). Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor. Biotechnology and bioengineering, 102(1), 100-112.
Miller, G. (1959). Determination of reducing sugar by DNS method. Anal. Chem, 31, 426-428.
Seo, H. B., Kim, H. J., Lee, O. K., Ha, J. H., Lee, H. Y., & Jung, K. H. (2009). Measurement of ethanol concentration using solvent extraction and dichromate oxidation and its application to bioethanol production process. Journal of industrial microbiology & biotechnology, 36(2), 285-292.
Pinheiro, R., Belo, I., & Mota, M. (2000). Air pressure effects on biomass yield of two different Kluyveromyces strains. Enzyme and microbial technology, 26(9-10), 756-762.
Jamieson, D. J. (1998). Oxidative stress responses of the yeast Saccharomyces cerevisiae. Yeast, 14(16), 1511-1527.
Verduyn, C. (1992). Physiology of yeasts in relation to biomass yields. In Quantitative Aspects of Growth and Metabolism of Microorganisms (pp. 325-353). Springer, Dordrecht.
Pérez-Torrado, R., Gómez-Pastor, R., Larsson, C., & Matallana, E. (2009). Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth. Applied microbiology and biotechnology, 81(5), 951-960.
Verduyn, C., Zomerdijk, T. P., van Dijken, J. P., & Scheffers, W. A. (1984). Continuous measurement of ethanol production by aerobic yeast suspensions with an enzyme electrode. Applied microbiology and biotechnology, 19(3), 181-185.
Otterstedt, K., Larsson, C., Bill, R. M., Ståhlberg, A., Boles, E., Hohmann, S., & Gustafsson, L. (2004). Switching the mode of metabolism in the yeast Saccharomyces cerevisiae. EMBO reports, 5(5), 532-537.
Chojnacka, K., & Marquez-Rocha, F. J. (2004). Kinetic and stoichiometric relationships of the energy and carbon metabolism in the culture of microalgae. Biotechnology, 3(1), 21-34.
Pope, D. H. (1975). Effects of light intensity, oxygen concentration, and carbon dioxide concentration on photosynthesis in algae. Microbial ecology, 2(1), 1-16.
Yen, H. W., Chen, P. W., & Chen, L. J. (2015). The synergistic effects for the co-cultivation of oleaginous yeast-Rhodotorula glutinis and microalgae-Scenedesmus obliquus on the biomass and total lipids accumulation. Bioresource technology, 184, 148-152.
Jia, H., & Yuan, Q. (2018). Ammonium removal using algae–bacteria consortia: the effect of ammonium concentration, algae biomass, and light. Biodegradation, 29(2), 105-115.
Cheng J. (2017). Biomass to Renewable Energy Processes. 2nd ed. CRC Press. 437.
Downloads
Published
How to Cite
Issue
Section
Categories
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © JTE.
